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SRX385627: GSM1277194: RNASeq_riboM_contDevB_N2_28hr; Caenorhabditis elegans; RNA-Seq
2 ILLUMINA (Illumina HiSeq 2000) runs: 54.8M spots, 2.8G bases, 1.8Gb downloads

Submitted by: Gene Expression Omnibus (GEO)
Study: Developmental Timecourses total-RNA sequencing [Ribosome repleted total RNA]
show Abstracthide Abstract
We report the presence of extensive, transcriptionally controlled oscillations in the C. elegans, developmental transcriptome. Furthermore, using ribosome profiling, we show that these oscillating transcripts are actively translated. Overall design: Examination of two timecourses that were collected over C. elegans development and analyzed by RNA-seq of "RiboMinus" libraries
Sample: RNASeq_riboM_contDevB_N2_28hr
SAMN02428714 • SRS509733 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: total RNA isolation was performed using Tri-Reagent (MRC) Library preparation was performed using the ScriptSeq v2 RNA-Seq library preparation kit (Epicentre) according to the manufacturer's protocol
Experiment attributes:
GEO Accession: GSM1277194
Links:
External link:
Runs: 2 runs, 54.8M spots, 2.8G bases, 1.8Gb
Run# of Spots# of BasesSizePublished
SRR104125050,000,0002.6G1.6Gb2014-03-03
SRR10412514,804,620245M163.5Mb2014-03-03

ID:
553479

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